Hotspot

trnT(GGU)-psbD

LSC · 35115-36243 · 1129 bp

Back to Typhonium

Pi 0.0151
MarkerSeek score 67.60
Primer available yes
Label trnT(GGU)-psbD

Feature Metadata

label_name
trnT(GGU)-psbD
feature_type
igs
region
LSC
length_bp
1129
variable_sites
17
pi
0.0151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(GGU)-psbD_p1 GTGGTAGAGTAACGCCATGG CACAAAAACGAAACGGTCCC 55.0 50.0 58.1 58.2 1280-1313 bp 1.0 79.3
trnT(GGU)-psbD_p2 GTGGTAGAGTAACGCCATGG CCACAAAAACGAAACGGTCC 55.0 50.0 58.1 58.2 1281-1314 bp 1.0 79.3
trnT(GGU)-psbD_p3 GTGGTAGAGTAACGCCATGG CCCACAAAAACGAAACGGTC 55.0 50.0 58.1 58.2 1282-1315 bp 1.0 79.3
trnT(GGU)-psbD_p4 TCAGTGGTAGAGTAACGCCA CACAAAAACGAAACGGTCCC 50.0 50.0 58.1 58.2 1283-1316 bp 1.0 79.1
trnT(GGU)-psbD_p5 TCAGTGGTAGAGTAACGCCA CCACAAAAACGAAACGGTCC 50.0 50.0 58.1 58.2 1284-1317 bp 1.0 79.1