Hotspot

rpoB-trnC(GCA)

LSC · 29027-30595 · 1569 bp

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Pi 0.0193
MarkerSeek score 62.70
Primer available yes
Label rpoB-trnC(GCA)

Feature Metadata

label_name
rpoB-trnC(GCA)
feature_type
igs
region
LSC
length_bp
1569
variable_sites
26
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC(GCA)_p1 CCCTCATTTTCATCCCGGAG AAAGGATTTGCAGTCCCCTG 55.0 50.0 58.0 58.1 1439-1635 bp 1.0 84.6
rpoB-trnC(GCA)_p2 CTCATTTTCATCCCGGAGCA AAAGGATTTGCAGTCCCCTG 50.0 50.0 58.0 58.1 1437-1633 bp 1.0 84.4
rpoB-trnC(GCA)_p3 CCCTCATTTTCATCCCGGAG CGACACCCAGACTTGAACTG 55.0 55.0 58.0 58.5 1464-1660 bp 1.0 81.1
rpoB-trnC(GCA)_p4 CTCATTTTCATCCCGGAGCA CGACACCCAGACTTGAACTG 50.0 55.0 58.0 58.5 1462-1658 bp 1.0 81.0
rpoB-trnC(GCA)_p5 CCCTCATTTTCATCCCGGAG CCTTACCACTTGGCCATGTC 55.0 55.0 58.0 58.5 1419-1615 bp 1.0 80.9