Hotspot

trnS(GCU)-trnS (CGA)

LSC · 9565-10947 · 1383 bp

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Pi 0.0851

MarkerSeek score 88.60

Primer available yes

Label trnS(GCU)-trnS (CGA)

Feature Metadata

label_name: trnS(GCU)-trnS (CGA)
feature_type: igs
region: LSC
length_bp: 1383
variable_sites: 104
pi: 0.0851
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnS (CGA)_p1	GAGAAAGCGGAGAAAGAGGG	TGGAATACCAACTGAAAGACCC	55.0	45.5	58.0	58.0	1559-1684 bp	1.0	72.8
trnS(GCU)-trnS (CGA)_p2	ATTAGCAATCCGCCGCTTTA	TGGAATACCAACTGAAAGACCC	45.0	45.5	58.0	58.0	1504-1629 bp	1.0	72.8
trnS(GCU)-trnS (CGA)_p3	TCAAGTGACCAGAGAAAGCG	TGGAATACCAACTGAAAGACCC	50.0	45.5	57.8	58.0	1570-1695 bp	1.0	71.6
trnS(GCU)-trnS (CGA)_p4	GAGAAAGCGGAGAAAGAGGG	GGAATACCAACTGAAAGACCCT	55.0	45.5	58.0	57.7	1558-1683 bp	1.0	70.6
trnS(GCU)-trnS (CGA)_p5	ATTAGCAATCCGCCGCTTTA	GGAATACCAACTGAAAGACCCT	45.0	45.5	58.0	57.7	1503-1628 bp	1.0	70.6