Hotspot

ndhF

SSC · 116120-118333 · 2214 bp

Pi 0.00569

MarkerSeek score 46.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 27
pi: 0.00569
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TTCTCTACGAAGTCGGGAGT	50.0	50.0	58.0	57.5	2785-2817 bp	1.0	77.6
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGTTCTCTACGAAGTCGGGA	50.0	50.0	58.0	57.5	2787-2819 bp	1.0	77.5
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGTTCTCTACGAAGTCGGGA	50.0	50.0	58.0	57.5	2779-2811 bp	1.0	77.5
ndhF_p4	AAGAGGGATCCACCGAAGAA	TTCTCTACGAAGTCGGGAGT	50.0	50.0	58.0	57.5	2777-2809 bp	1.0	77.5
ndhF_p5	ACAGGAACAAGAGGGATCCA	CAGTTCTCTACGAAGTCGGG	50.0	55.0	58.0	57.5	2788-2820 bp	1.0	77.1