Hotspot

rps15-ycf1

SSC · 129759-130215 · 457 bp

Back to Typha

Pi 0.0328
MarkerSeek score 66.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
457
variable_sites
24
pi
0.0328
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GGTGCTGAGGACCCATATTG TTCTTTGGCCCAATTACCGA 55.0 45.0 58.0 57.4 990-1030 bp 1.0 79.5
rps15-ycf1_p2 AACACCATGCATTGATGGGT TTCTTTGGCCCAATTACCGA 45.0 45.0 58.1 57.4 1007-1047 bp 1.0 79.2
rps15-ycf1_p3 TCAGTCGAAGAACACCATGC TTCTTTGGCCCAATTACCGA 50.0 45.0 58.2 57.4 1017-1057 bp 1.0 78.0
rps15-ycf1_p4 TCCGCCATAAAAGGTCCAAG TTCTTTGGCCCAATTACCGA 50.0 45.0 57.9 57.4 1303-1343 bp 1.0 77.7
rps15-ycf1_p5 GGTGCTGAGGACCCATATTG AGAGGAGAGATGCATCTGGA 55.0 50.0 58.0 57.2 1480-1520 bp 1.0 77.1