Hotspot

rpoB-trnC-GCA

LSC · 28130-29174 · 1045 bp

Back to Typha

Pi 0.00792
MarkerSeek score 64.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1045
variable_sites
17
pi
0.00792
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TGCCGTCAAGCCTTGATTAA AAAGGATTTGCAGTCCCCTG 45.0 50.0 57.8 58.1 1137-1187 bp 1.0 82.5
rpoB-trnC-GCA_p2 CTGAAATTCTGCCGTCAAGC AAAGGATTTGCAGTCCCCTG 50.0 50.0 57.7 58.1 1146-1196 bp 1.0 82.0
rpoB-trnC-GCA_p3 CTGCCGTCAAGCCTTGATTA AAAGGATTTGCAGTCCCCTG 50.0 50.0 58.3 58.1 1138-1188 bp 1.0 81.9
rpoB-trnC-GCA_p4 TCCCTCATTTCCATTCCGGA AAAGGATTTGCAGTCCCCTG 50.0 50.0 58.4 58.1 1062-1112 bp 1.0 81.1
rpoB-trnC-GCA_p5 AAATTCTGCCGTCAAGCCTT AAAGGATTTGCAGTCCCCTG 45.0 50.0 58.4 58.1 1143-1193 bp 1.0 81.0