Hotspot

trnS-GCU-trnG-UCC

LSC · 8480-10117 · 1638 bp

Back to Typha

Pi 0.0308
MarkerSeek score 79.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1638
variable_sites
98
pi
0.0308
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 1585-1800 bp 1.0 60.0
trnS-GCU-trnG-UCC_p2 TAGGGAACGGAAAGAGAGGG ACGAATCACACTTTTACCACT 55.0 38.1 58.1 55.5 1640-1855 bp 1.0 59.1
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 1634-1849 bp 1.0 59.0
trnS-GCU-trnG-UCC_p4 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 1588-1803 bp 1.0 59.0
trnS-GCU-trnG-UCC_p5 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 1587-1802 bp 1.0 59.0