Hotspot

psbI-trnS-GCU

LSC · 8262-8391 · 130 bp

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Pi 0.02
MarkerSeek score 65.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
130
variable_sites
5
pi
0.02
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAGGA TAAAGCGGCGGATTGCTAAT 55.0 45.0 58.9 58.0 221-224 bp 1.0 74.7
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAGGA ATTGGGAGAGATGGCTGAGT 55.0 50.0 58.9 58.1 245-248 bp 1.0 73.9
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAGGA ATGGCTGAGTGGACTAAAGC 55.0 50.0 58.9 57.6 235-238 bp 1.0 71.6
psbI-trnS-GCU_p4 GTAATCCTGGACGTGAGGAA TAAAGCGGCGGATTGCTAAT 50.0 45.0 56.7 58.0 220-223 bp 1.0 71.1
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAGGA GAGAGATGGCTGAGTGGACT 55.0 55.0 58.9 58.5 240-243 bp 1.0 70.7