Hotspot

ycf3

LSC · 46238-48207 · 1970 bp

Back to Turpinia

Pi 0.00102

MarkerSeek score 58.40

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1970
variable_sites: 2
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TTGATTGATGGGGCCAACAA	TGGGATCATTTTCTCACGAGAG	45.0	45.5	58.0	57.9	2090 bp	1.0	62.9
ycf3_p2	TTTGATTGATGGGGCCAACA	TGGGATCATTTTCTCACGAGAG	45.0	45.5	58.0	57.9	2091 bp	1.0	62.9
ycf3_p3	TTGATTGATGGGGCCAACAA	GGATCATTTTCTCACGAGAGGT	45.0	45.5	58.0	57.9	2088 bp	1.0	62.9
ycf3_p4	TTTGATTGATGGGGCCAACA	GGATCATTTTCTCACGAGAGGT	45.0	45.5	58.0	57.9	2089 bp	1.0	62.9
ycf3_p5	TTGATTGATGGGGCCAACAA	GTGGGATCATTTTCTCACGAGA	45.0	45.5	58.0	58.1	2091 bp	1.0	62.8