Hotspot

clpP

LSC · 75444-77550 · 2107 bp

Back to Turpinia

Pi 0.00332

MarkerSeek score 59.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2107
variable_sites: 7
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATCGATCCTAACCGGACGAT	ACGCTTCCACATCAAAGTGA	50.0	45.0	58.1	57.7	2279-2281 bp	1.0	77.5
clpP_p2	ATCGATCCTAACCGGACGAT	AACCCATTGTTACGCTTCCA	50.0	45.0	58.1	57.7	2290-2292 bp	1.0	77.3
clpP_p3	ATCGATCCTAACCGGACGAT	ACCCATTGTTACGCTTCCAC	50.0	50.0	58.1	58.5	2289-2291 bp	1.0	75.7
clpP_p4	ATCGATCCTAACCGGACGAT	CCCATTGTTACGCTTCCACA	50.0	50.0	58.1	58.5	2288-2290 bp	1.0	75.7
clpP_p5	TCGATCCTAACCGGACGATT	ACGCTTCCACATCAAAGTGA	50.0	45.0	58.3	57.7	2278-2280 bp	1.0	75.7