Hotspot

cemA

LSC · 66072-66767 · 696 bp

Back to Turpinia

Pi 0.00144

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 696
variable_sites: 1
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGATCGCATAGAGTCGACGA	CCTGGATGGGTAGAAAGAGT	50.0	50.0	58.1	55.9	835 bp	1.0	62.4
cemA_p2	CGCATAGAGTCGACGAATGA	CCTGGATGGGTAGAAAGAGT	50.0	50.0	57.6	55.9	831 bp	1.0	60.4
cemA_p3	TCGCATAGAGTCGACGAATG	CCTGGATGGGTAGAAAGAGT	50.0	50.0	57.6	55.9	832 bp	1.0	60.4
cemA_p4	GACGAATGAGGTGGGTTCAT	CCTGGATGGGTAGAAAGAGT	50.0	50.0	57.6	55.9	820 bp	1.0	60.2
cemA_p5	GATCGCATAGAGTCGACGAA	CCTGGATGGGTAGAAAGAGT	50.0	50.0	57.3	55.9	834 bp	1.0	58.1