Hotspot

accD-psaI

LSC · 63358-64052 · 695 bp

Back to Turpinia

Pi 0.00144

MarkerSeek score 59.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 1
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTATTTCAGCTCCACGCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	821-826 bp	1.0	77.9
accD-psaI_p2	GTTATTTCAGCTCCACGCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	820-825 bp	1.0	77.9
accD-psaI_p3	AGTTATTTCAGCTCCACGCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	827-832 bp	1.0	77.3
accD-psaI_p4	GTTATTTCAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	826-831 bp	1.0	77.3
accD-psaI_p5	ATTTCAGCTCCACGCCTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.4	58.0	817-822 bp	1.0	76.9