Hotspot

psbI-trnS-GCU

LSC · 9062-9263 · 202 bp

Back to Turpinia

Pi 0.0101

MarkerSeek score 60.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 2
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.6	58.0	294-298 bp	1.0	78.4
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.6	58.0	295-299 bp	1.0	78.4
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.6	58.1	313-317 bp	1.0	77.5
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.6	58.4	292-296 bp	1.0	75.6
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.6	58.4	299-303 bp	1.0	75.4