Hotspot

rps8-rpl14

LSC · 82070-82238 · 169 bp

Back to Tupistra

Pi 0.00592

MarkerSeek score 55.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 1
pi: 0.00592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GTGGATGCTATTCGAACCGT	CGATTGCTCGGGAATTGAGA	50.0	50.0	58.1	58.1	323 bp	1.0	79.3
rps8-rpl14_p2	GTGGATGCTATTCGAACCGT	TTGCTCGGGAATTGAGACAG	50.0	50.0	58.1	57.9	320 bp	1.0	78.9
rps8-rpl14_p3	GTGGATGCTATTCGAACCGT	GCTCGGGAATTGAGACAGTT	50.0	50.0	58.1	57.9	318 bp	1.0	78.9
rps8-rpl14_p4	AGTGGATGCTATTCGAACCG	CGATTGCTCGGGAATTGAGA	50.0	50.0	57.8	58.1	324 bp	1.0	78.0
rps8-rpl14_p5	AGTGGATGCTATTCGAACCG	TTGCTCGGGAATTGAGACAG	50.0	50.0	57.8	57.9	321 bp	1.0	77.7