Hotspot

ndhC-trnV-UAC

LSC · 51112-52629 · 1518 bp

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Pi 0
MarkerSeek score 59.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1518
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGTCCTTCACTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.8 57.8 1654-1664 bp 1.0 77.1
ndhC-trnV-UAC_p2 GGTCCTTCACTAATCGGAGC AGCCCAATGCAACAAAATGC 55.0 45.0 57.8 58.5 1736-1746 bp 1.0 74.9
ndhC-trnV-UAC_p3 GGTCCTTCACTAATCGGAGC GAAGGTCTACGGTTCGAGTC 55.0 55.0 57.8 57.5 1653-1663 bp 1.0 74.3
ndhC-trnV-UAC_p4 GGTCCTTCACTAATCGGAGC GTTCGAGTCCGTATAGCCCT 55.0 55.0 57.8 58.7 1642-1652 bp 1.0 73.2
ndhC-trnV-UAC_p5 GGTCCTTCACTAATCGGAGC GAAAGAGCCCAATGCAACAA 55.0 45.0 57.8 57.2 1741-1751 bp 1.0 72.1