Hotspot

trnQ-UUG-psbK

LSC · 6885-7229 · 345 bp

Back to Tupistra

Pi 0.0058

MarkerSeek score 56.80

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 2
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CTGATGGAGACGATCTGGGA	TCGGGCAATTTAGCGAAGAA	55.0	45.0	58.3	57.9	514 bp	1.0	77.1
trnQ-UUG-psbK_p2	TCTGATGGAGACGATCTGGG	TCGGGCAATTTAGCGAAGAA	55.0	45.0	58.3	57.9	515 bp	1.0	77.1
trnQ-UUG-psbK_p3	CTGATGGAGACGATCTGGGA	CTCGGGCAATTTAGCGAAGA	55.0	50.0	58.3	58.3	515 bp	1.0	75.1
trnQ-UUG-psbK_p4	TCTGATGGAGACGATCTGGG	CTCGGGCAATTTAGCGAAGA	55.0	50.0	58.3	58.3	516 bp	1.0	75.1
trnQ-UUG-psbK_p5	TGATGGAGACGATCTGGGAC	TCGGGCAATTTAGCGAAGAA	55.0	45.0	58.6	57.9	513 bp	1.0	74.7