Hotspot

matK-trnK-UUU

LSC · 3228-4011 · 784 bp

Back to Tupistra

Pi 0.00128

MarkerSeek score 59.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 1
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TAGGAAGTGTTGTTGCCGAG	TCTGGGTTGCTAACTCAACG	50.0	50.0	57.8	57.8	881 bp	1.0	77.5
matK-trnK-UUU_p2	TAGGAAGTGTTGTTGCCGAG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.8	58.2	865 bp	1.0	77.4
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCCGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	864 bp	1.0	77.4
matK-trnK-UUU_p4	TAGGAAGTGTTGTTGCCGAG	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.8	57.5	878 bp	1.0	75.1
matK-trnK-UUU_p5	GGAAGTGTTGTTGCCGAGAT	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.5	57.8	879 bp	1.0	75.0