Hotspot

ycf1

SSC · 122047-127353 · 5307 bp

Back to Tulipa

Pi 0.00983
MarkerSeek score 49.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5307
variable_sites
471
pi
0.00983
unique_haplotype_count
69
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTCGGGACTAATCGAGACCA ACAGAACGTCCCTTTGGTTC 50.0 50.0 57.9 58.0 1061-1115 bp 1.0 87.2
ycf1_p2 TTCGGGACTAATCGAGACCA CCTTTGGTTCTCCCCGAAAA 50.0 50.0 57.9 58.0 1051-1105 bp 0.885057 82.9
ycf1_p3 TCGGGACTAATCGAGACCAT CCTTTGGTTCTCCCCGAAAA 50.0 50.0 57.6 58.0 1050-1104 bp 0.885057 81.0
ycf1_p4 TTCGGGACTAATCGAGACCA GGTTCTCCCCGAAAACAACT 50.0 50.0 57.9 58.0 1046-1082 bp 0.724138 76.3
ycf1_p5 TCGGGACTAATCGAGACCAT GGTTCTCCCCGAAAACAACT 50.0 50.0 57.6 58.0 1045-1081 bp 0.724138 74.5