Hotspot

psbA

LSC · 135-1196 · 1062 bp

Back to Tulipa

Pi 0.00231
MarkerSeek score 31.40
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
26
pi
0.00231
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TTGCTCTGTCAACGATTCGT GCGTGCTCTTTTGCAAAGAT 45.0 45.0 57.9 57.9 1817-1880 bp 1.0 84.1
psbA_p2 TGGGTATTGCTCTGTCAACG GCGTGCTCTTTTGCAAAGAT 50.0 45.0 57.9 57.9 1823-1886 bp 0.977011 83.3
psbA_p3 TTGCTCTGTCAACGATTCGT GTGCTTGGGAGTCCTTGAAA 45.0 50.0 57.9 58.0 1143-1180 bp 1.0 81.3
psbA_p4 TGGGTATTGCTCTGTCAACG GTGCTTGGGAGTCCTTGAAA 50.0 50.0 57.9 58.0 1149-1186 bp 0.977011 80.6
psbA_p5 TGGGTATTGCTCTGTCAACG AAATGCAAGCACGATTTGGG 50.0 45.0 57.9 57.9 1437-1474 bp 0.977011 80.5