Hotspot

ndhD

SSC · 113743-115248 · 1506 bp

Back to Tulipa

Pi 0.00526
MarkerSeek score 41.50
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1506
variable_sites
74
pi
0.00526
unique_haplotype_count
38
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 ACAGAATTATAAGACTTTCCGTC GTACGGGCTTTTCTGGTCAA 34.8 50.0 54.2 58.1 1578 bp 0.988506 43.4
ndhD_p2 ACAGAATTATAAGACTTTCCGTC CAAGTACGGGCTTTTCTGGT 34.8 50.0 54.2 58.1 1581 bp 0.977011 42.3
ndhD_p3 GACTTTCCGTCTCATTAATAAACA GTACGGGCTTTTCTGGTCAA 33.3 50.0 55.2 58.1 1566 bp 0.954023 41.8
ndhD_p4 GACTTTCCGTCTCATTAATAAACA CAAGTACGGGCTTTTCTGGT 33.3 50.0 55.2 58.1 1569 bp 0.942529 40.7
ndhD_p5 GACTTTCCGTCTCATTAATAAACA TCAAGTACGGGCTTTTCTGG 33.3 50.0 55.2 57.8 1570 bp 0.942529 40.7