Hotspot

ccsA-ndhD

SSC · 113561-113742 · 182 bp

Back to Tulipa

Pi 0.0534
MarkerSeek score 64.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
182
variable_sites
43
pi
0.0534
unique_haplotype_count
34
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGGCACTTGCAGCTTCTATA TCAGTAGCTTTTCGATGGCC 50.0 50.0 58.1 58.0 613-681 bp 1.0 91.3
ccsA-ndhD_p2 CGGCACTTGCAGCTTCTATA CAGTAGCTTTTCGATGGCCT 50.0 50.0 58.1 58.0 612-680 bp 1.0 91.3
ccsA-ndhD_p3 CGGCACTTGCAGCTTCTATA GATAGCTGTTCCGATGCCAA 50.0 50.0 58.1 58.0 648-716 bp 1.0 91.1
ccsA-ndhD_p4 GCTAATGAGGCTTGGGGATC TCAGTAGCTTTTCGATGGCC 55.0 50.0 58.1 58.0 737-805 bp 1.0 90.7
ccsA-ndhD_p5 CGGCACTTGCAGCTTCTATA CTATGCGGCTTCAACCTCTT 50.0 50.0 58.1 58.0 879-947 bp 1.0 90.3