Hotspot

rpl14-rpl16

LSC · 79550-79680 · 131 bp

Back to Tulipa

Pi 0.0373
MarkerSeek score 69.70
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
131
variable_sites
28
pi
0.0373
unique_haplotype_count
36
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 CGAGCTCCACTATTATCCGC GGGCGGAGTATCAGAAACTG 55.0 55.0 58.0 58.1 263-291 bp 1.0 91.4
rpl14-rpl16_p2 CGAGCTCCACTATTATCCGC TGCGCGTAAGATGCCTATAC 55.0 50.0 58.0 57.9 218-246 bp 1.0 91.4
rpl14-rpl16_p3 TCGTTGGTTACCAGCTCCTA GGGCGGAGTATCAGAAACTG 50.0 55.0 58.1 58.1 306-334 bp 1.0 91.0
rpl14-rpl16_p4 TCGTTGGTTACCAGCTCCTA TGCGCGTAAGATGCCTATAC 50.0 50.0 58.1 57.9 261-289 bp 1.0 90.9
rpl14-rpl16_p5 CGAGCTCCACTATTATCCGC ACTGTAGCCAGAGCAGCTAT 55.0 50.0 58.0 58.2 247-275 bp 1.0 90.2