Hotspot

rps8-rpl14

LSC · 78990-79180 · 191 bp

Back to Tulipa

Pi 0.0262
MarkerSeek score 63.40
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
191
variable_sites
34
pi
0.0262
unique_haplotype_count
39
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 AGATGCTATTCGAACCGTTCT AATCGCTCGGGAATTGAGAC 42.9 50.0 57.2 58.1 335-373 bp 1.0 76.3
rps8-rpl14_p2 AGATGCTATTCGAACCGTTCT CAATCGCTCGGGAATTGAGA 42.9 50.0 57.2 58.1 336-374 bp 1.0 76.3
rps8-rpl14_p3 AGATGCTATTCGAACCGTTCT GCTCGGGAATTGAGACAGTT 42.9 50.0 57.2 57.9 331-369 bp 0.988506 75.6
rps8-rpl14_p4 CGGCAATAGTATCCTTACCCA AATCGCTCGGGAATTGAGAC 47.6 50.0 57.0 58.1 273-311 bp 1.0 75.1
rps8-rpl14_p5 CGGCAATAGTATCCTTACCCA CAATCGCTCGGGAATTGAGA 47.6 50.0 57.0 58.1 274-312 bp 1.0 75.1