Hotspot

ycf2-trnH-GUG

LSC · 119846-120281 · 436 bp

Back to Tsuga

Pi 0.0216
MarkerSeek score 52.50
Primer available yes
Label ycf2-trnH-GUG

Feature Metadata

label_name
ycf2-trnH-GUG
feature_type
igs
region
LSC
length_bp
436
variable_sites
14
pi
0.0216
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.73
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnH-GUG_p1 TTTATAAGGCGCTCCCGATG GTAGTTCGGGACCCACATTC 50.0 55.0 57.8 58.0 683-863 bp 1.0 84.5
ycf2-trnH-GUG_p2 TTTATAAGGCGCTCCCGATG TAGTTCGGGACCCACATTCT 50.0 50.0 57.8 58.1 682-862 bp 1.0 84.3
ycf2-trnH-GUG_p3 TTTATAAGGCGCTCCCGATG ACATTTGACAGAGGTCCACG 50.0 50.0 57.8 57.8 628-808 bp 1.0 83.2
ycf2-trnH-GUG_p4 TTTATAAGGCGCTCCCGATG CTTGCTTTGATCCCCTGTCA 50.0 50.0 57.8 57.8 564-744 bp 1.0 82.9
ycf2-trnH-GUG_p5 TTTATAAGGCGCTCCCGATG TTGCTTTGATCCCCTGTCAG 50.0 50.0 57.8 57.8 563-743 bp 1.0 82.9