Hotspot

ycf3-psaA

LSC · 71712-72566 · 855 bp

Back to Tsuga

Pi 0.0133
MarkerSeek score 72.00
Primer available yes
Label ycf3-psaA

Feature Metadata

label_name
ycf3-psaA
feature_type
igs
region
LSC
length_bp
855
variable_sites
23
pi
0.0133
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-psaA_p1 CTCCGGGCAATTACATCGAA CCAGGTTTGGCCCATTTTTC 50.0 50.0 58.1 57.8 943-1018 bp 1.0 84.0
ycf3-psaA_p2 TCCGGGCAATTACATCGAAG CCAGGTTTGGCCCATTTTTC 50.0 50.0 58.1 57.8 942-1017 bp 1.0 84.0
ycf3-psaA_p3 GCTGCTGAGTATTGGGAACA CCAGGTTTGGCCCATTTTTC 50.0 50.0 57.9 57.8 977-1052 bp 1.0 83.4
ycf3-psaA_p4 CTCCGGGCAATTACATCGAA CGGCCTTTACGTCTAGTTGT 50.0 50.0 58.1 57.6 891-966 bp 1.0 82.7
ycf3-psaA_p5 TCCGGGCAATTACATCGAAG CGGCCTTTACGTCTAGTTGT 50.0 50.0 58.1 57.6 890-965 bp 1.0 82.7