Hotspot

trnS-GGA-ycf3

LSC · 69028-69769 · 742 bp

Back to Tsuga

Pi 0.00339
MarkerSeek score 63.20
Primer available yes
Label trnS-GGA-ycf3

Feature Metadata

label_name
trnS-GGA-ycf3
feature_type
igs
region
LSC
length_bp
742
variable_sites
5
pi
0.00339
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-ycf3_p1 GAACCCTCGGTAAGCAGAAG CGGAGCCATCGGGATTATTC 55.0 55.0 58.0 58.3 890-902 bp 1.0 80.3
trnS-GGA-ycf3_p2 GAACCCTCGGTAAGCAGAAG GGAGCCATCGGGATTATTCG 55.0 55.0 58.0 58.3 889-901 bp 1.0 80.3
trnS-GGA-ycf3_p3 ACGGAAAGAGAGGGATTCGA CGGAGCCATCGGGATTATTC 50.0 55.0 58.2 58.3 908-920 bp 1.0 79.1
trnS-GGA-ycf3_p4 ACGGAAAGAGAGGGATTCGA GGAGCCATCGGGATTATTCG 50.0 55.0 58.2 58.3 907-919 bp 1.0 79.1
trnS-GGA-ycf3_p5 TTCCAATGCTACGCCTTGAA GGAGCCATCGGGATTATTCG 45.0 55.0 57.8 58.3 857-869 bp 1.0 79.0