Hotspot

trnL-UAA-trnT-UGU

LSC · 67315-67738 · 424 bp

Back to Tsuga

Pi 0.00283
MarkerSeek score 62.40
Primer available yes
Label trnL-UAA-trnT-UGU

Feature Metadata

label_name
trnL-UAA-trnT-UGU
feature_type
igs
region
LSC
length_bp
424
variable_sites
3
pi
0.00283
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnT-UGU_p1 ATTCGATCAAGTCCGTAGCG CAAGTGCGATGCTCTAACCT 50.0 50.0 57.9 58.0 433-502 bp 1.0 83.4
trnL-UAA-trnT-UGU_p2 CCGTAGCGTCTACCAATTCC CAAGTGCGATGCTCTAACCT 55.0 50.0 58.2 58.0 421-490 bp 1.0 83.2
trnL-UAA-trnT-UGU_p3 ATTCGATCAAGTCCGTAGCG GAACGGAAAAGAGCCAGCTA 50.0 50.0 57.9 57.9 482-551 bp 1.0 82.7
trnL-UAA-trnT-UGU_p4 ATTCGATCAAGTCCGTAGCG GCATTACAAGTGCGATGCTC 50.0 50.0 57.9 57.9 439-508 bp 1.0 82.7
trnL-UAA-trnT-UGU_p5 ATTCGATCAAGTCCGTAGCG ATGGGAATGAGAGGAACGGA 50.0 50.0 57.9 58.1 495-564 bp 1.0 82.5