Hotspot

trnH-GUG-trnV-UAC

SSC · 30085-31756 · 1672 bp

Back to Tsuga

Pi 0.0087
MarkerSeek score 61.40
Primer available yes
Label trnH-GUG-trnV-UAC

Feature Metadata

label_name
trnH-GUG-trnV-UAC
feature_type
igs
region
SSC
length_bp
1672
variable_sites
32
pi
0.0087
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-trnV-UAC_p1 TTCACAATCCACTGCCTTGG CATGTTGGGTTCCTGGAACA 50.0 50.0 58.4 58.0 1442-1850 bp 1.0 82.4
trnH-GUG-trnV-UAC_p2 TTCACAATCCACTGCCTTGG TGCGAGAAAATGCATGTTGG 50.0 45.0 58.4 57.6 1454-1862 bp 1.0 79.6
trnH-GUG-trnV-UAC_p3 TTCACAATCCACTGCCTTGG GCGAGAAAATGCATGTTGGG 50.0 50.0 58.4 58.4 1453-1861 bp 1.0 79.5
trnH-GUG-trnV-UAC_p4 CGTGTGGTGGATTCACAATC CATGTTGGGTTCCTGGAACA 50.0 50.0 57.1 58.0 1453-1861 bp 1.0 78.2
trnH-GUG-trnV-UAC_p5 ATTTTATGGGCGAACGACGG CATGTTGGGTTCCTGGAACA 50.0 50.0 59.0 58.0 1485-1893 bp 1.0 77.5