Hotspot

rpl32-trnL-UAG

SSC · 118882-119704 · 823 bp

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Pi 0.0182

MarkerSeek score 57.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 823
variable_sites: 15
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTGTTTCCACTGGACAGTCA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	57.4	58.0	1529-1531 bp	1.0	76.2
rpl32-trnL-UAG_p2	CTGTTTCCACTGGACAGTCA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	57.4	58.0	1528-1530 bp	1.0	76.2
rpl32-trnL-UAG_p3	CTGTTTCCACTGGACAGTCA	GTCCCGAAGAAATCCAACGA	50.0	50.0	57.4	57.9	1262-1264 bp	1.0	76.2
rpl32-trnL-UAG_p4	CTGTTTCCACTGGACAGTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.4	57.9	927-934 bp	1.0	75.5
rpl32-trnL-UAG_p5	CTGTTTCCACTGGACAGTCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.4	57.8	920-927 bp	1.0	75.0