Hotspot

ndhF-rpl32

SSC · 117885-118707 · 823 bp

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Pi 0.0458
MarkerSeek score 77.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
823
variable_sites
37
pi
0.0458
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGACATAGTAACTGGGAGCG TCCAAAGATTTCTACGAATATGCT 55.0 33.3 57.9 56.3 947-951 bp 1.0 44.5
ndhF-rpl32_p2 GGAGCGGAAAAAGGGGTATT TCCAAAGATTTCTACGAATATGCT 50.0 33.3 57.9 56.3 933-937 bp 1.0 44.5
ndhF-rpl32_p3 ACATAGTAACTGGGAGCGGA TCCAAAGATTTCTACGAATATGCT 50.0 33.3 57.8 56.3 945-949 bp 1.0 44.5
ndhF-rpl32_p4 TAACTGGGAGCGGAAAAAGG TCCAAAGATTTCTACGAATATGCT 50.0 33.3 57.8 56.3 939-943 bp 1.0 44.5
ndhF-rpl32_p5 CGACATAGTAACTGGGAGCG TCCAAAGATTTCTACGAATATGCTT 55.0 32.0 57.9 56.9 947-951 bp 1.0 44.5