Hotspot

petD-rpoA

LSC · 82051-82205 · 155 bp

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Pi 0.0203

MarkerSeek score 68.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 3
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTACCGCAGTAGCTCTTTG	ATTGGGCACTCTACAGAAGC	55.0	50.0	58.4	57.6	326-333 bp	1.0	76.3
petD-rpoA_p2	GGTACCGCAGTAGCTCTTTG	GGGCACTCTACAGAAGCATT	55.0	50.0	58.4	57.6	323-330 bp	1.0	76.3
petD-rpoA_p3	GTACCGCAGTAGCTCTTTGG	ATTGGGCACTCTACAGAAGC	55.0	50.0	58.4	57.6	325-332 bp	1.0	76.3
petD-rpoA_p4	GTACCGCAGTAGCTCTTTGG	GGGCACTCTACAGAAGCATT	55.0	50.0	58.4	57.6	322-329 bp	1.0	76.3
petD-rpoA_p5	CCGCAGTAGCTCTTTGGTTA	GGGCACTCTACAGAAGCATT	50.0	50.0	57.6	57.6	319-326 bp	1.0	76.2