Hotspot

atpB-rbcL

LSC · 57606-58321 · 716 bp

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Pi 0.0168

MarkerSeek score 68.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 12
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	864-871 bp	1.0	79.7
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	832-839 bp	1.0	79.1
atpB-rbcL_p3	CGATACGCCCTAGGTTTTGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	815-822 bp	1.0	77.8
atpB-rbcL_p4	ATCTTGCCCGGTGGAAAAAT	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.1	58.4	865-872 bp	1.0	77.2
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	833-840 bp	1.0	76.7