Hotspot

ndhC-trnV-UAC

LSC · 53234-54623 · 1390 bp

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Pi 0.00873

MarkerSeek score 64.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1390
variable_sites: 12
pi: 0.00873
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTGGACCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	1526-1539 bp	1.0	78.2
ndhC-trnV-UAC_p2	TCTGGACCTTCCCTAATCGG	AGCCCAATGCAACAAAATGC	55.0	45.0	58.2	58.5	1608-1621 bp	1.0	75.9
ndhC-trnV-UAC_p3	TCTGGACCTTCCCTAATCGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.2	57.5	1525-1538 bp	1.0	75.5
ndhC-trnV-UAC_p4	TCTGGACCTTCCCTAATCGG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.2	58.7	1514-1527 bp	1.0	74.3
ndhC-trnV-UAC_p5	TCTGGACCTTCCCTAATCGG	GAAAGAGCCCAATGCAACAA	55.0	45.0	58.2	57.2	1613-1626 bp	1.0	73.2