Hotspot

ycf1

SSC · 122001-127493 · 5493 bp

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Pi 0.0591

MarkerSeek score 68.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 323
pi: 0.0591
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGGGGATATCCACCAGCAT	ATAGAGCCACCCAACACTGT	50.0	50.0	58.2	58.6	268 bp	1.0	80.8
ycf1_p2	ATGGGGATATCCACCAGCAT	AATAGAGCCACCCAACACTG	50.0	50.0	58.2	57.5	269 bp	0.5	54.1
ycf1_p3	ATGGGGATATCCACCAGCAT	CTGCCCTTCAATATCACGGA	50.0	50.0	58.2	57.4	475 bp	0.5	53.2
ycf1_p4	ATGGGGATATCCACCAGCAT	TCTGCCCTTCAATATCACGG	50.0	50.0	58.2	57.4	476 bp	0.5	53.2
ycf1_p5	ATGGGGATATCCACCAGCAT	GCAAAATAGAGCCACCCAAC	50.0	50.0	58.2	57.3	273 bp	0.5	52.9