Hotspot

rpl32-trnL-UAG

SSC · 111543-112470 · 928 bp

Back to Tropaeolum

Pi 0.0936

MarkerSeek score 84.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 928
variable_sites: 83
pi: 0.0936
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATGTGAATGGGGATGGGGAT	TTTAGTGGGGTCCTCCGATT	50.0	50.0	58.5	58.0	1565 bp	0.5	56.1
rpl32-trnL-UAG_p2	ATGTGAATGGGGATGGGGAT	TTTTAGTGGGGTCCTCCGAT	50.0	50.0	58.5	58.0	1566 bp	0.5	56.1
rpl32-trnL-UAG_p3	ATGTGAATGGGGATGGGGAT	ACTGAGATTGGAGAGCAGGT	50.0	50.0	58.5	58.1	1982 bp	0.5	56.0
rpl32-trnL-UAG_p4	ATGTGAATGGGGATGGGGAT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.5	57.9	1438 bp	0.5	55.4
rpl32-trnL-UAG_p5	ATGTGAATGGGGATGGGGAT	GACCCGAAGTAGCAAAACCT	50.0	50.0	58.5	57.8	1928 bp	0.5	55.0