Hotspot

trnP-UGG-psaJ

LSC · 64946-65289 · 344 bp

Back to Tropaeolum

Pi 0.0539

MarkerSeek score 79.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 18
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	CCTGCTAAAGAACCGAACCA	45.0	50.0	57.9	57.8	450-456 bp	1.0	82.6
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	ACCTGCTAAAGAACCGAACC	45.0	50.0	57.9	57.8	451-457 bp	1.0	82.6
trnP-UGG-psaJ_p3	AAGGATCCCTCGTTACTGCT	CCTGCTAAAGAACCGAACCA	50.0	50.0	58.1	57.8	527-533 bp	1.0	81.7
trnP-UGG-psaJ_p4	AAGGATCCCTCGTTACTGCT	ACCTGCTAAAGAACCGAACC	50.0	50.0	58.1	57.8	528-534 bp	1.0	81.7
trnP-UGG-psaJ_p5	ATCCCTCGTTACTGCTCAGA	CCTGCTAAAGAACCGAACCA	50.0	50.0	57.9	57.8	523-529 bp	1.0	81.7