Hotspot

psbE-petL

LSC · 62882-64119 · 1238 bp

Back to Tropaeolum

Pi 0.0602

MarkerSeek score 77.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 72
pi: 0.0602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCAGCCATTTATCCCGCTTC	TGACCTCCCGCGAATTAAAG	50.0	50.0	58.0	58.0	2335-2343 bp	1.0	84.3
psbE-petL_p2	TCAGCCATTTATCCCGCTTC	GCGGTAAGGAACTGTCGAAT	50.0	50.0	58.0	58.0	2070 bp	0.5	59.7
psbE-petL_p3	TCGTTCGGGTTTGATTGTGT	GCGGTAAGGAACTGTCGAAT	45.0	50.0	58.0	58.0	1827 bp	0.5	59.6
psbE-petL_p4	ATGGAATTCCTTGTCGGCTC	GCGGTAAGGAACTGTCGAAT	50.0	50.0	58.0	58.0	1596 bp	0.5	59.6
psbE-petL_p5	CGGTAGGTACAGCTAGTCCA	GCGGTAAGGAACTGTCGAAT	55.0	50.0	58.0	58.0	1737 bp	0.5	59.6