Hotspot

trnF-GAA-ndhJ

LSC · 47040-47601 · 562 bp

Back to Tropaeolum

Pi 0.0625

MarkerSeek score 81.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 29
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	579-674 bp	1.0	87.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	578-673 bp	1.0	87.0
trnF-GAA-ndhJ_p3	TGATGCTTCGGTAATGGTCG	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	634-729 bp	1.0	86.6
trnF-GAA-ndhJ_p4	TGATGCTTCGGTAATGGTCG	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	633-728 bp	1.0	86.6
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.2	58.0	620-715 bp	1.0	85.5