Hotspot

trnC-GCA-petN

LSC · 27163-27825 · 663 bp

Back to Tropaeolum

Pi 0.0553

MarkerSeek score 78.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 36
pi: 0.0553
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	719-741 bp	1.0	83.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	737-759 bp	1.0	81.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	718-740 bp	1.0	77.5
trnC-GCA-petN_p4	CCAGTTCAAATCCGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.8	58.2	714-736 bp	1.0	77.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	727-749 bp	1.0	77.3