Hotspot

trnS-GCU-trnG-UCC

LSC · 7769-8299 · 531 bp

Back to Tropaeolum

Pi 0.0667

MarkerSeek score 79.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 34
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GGGCGAATCACACTTTTACC	50.0	50.0	58.0	57.1	602-642 bp	1.0	79.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GGGCGAATCACACTTTTACC	50.0	50.0	58.0	57.1	601-641 bp	1.0	79.7
trnS-GCU-trnG-UCC_p3	AGGAAACGGAAAGAGAGGGA	GGGCGAATCACACTTTTACC	50.0	50.0	58.0	57.1	661-701 bp	1.0	78.9
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	GGGCGAATCACACTTTTACC	50.0	50.0	58.2	57.1	656-696 bp	1.0	78.0
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GGCGAATCACACTTTTACCAC	50.0	47.6	58.0	57.8	601-641 bp	1.0	77.5