Hotspot

trnK-UUU-rps16

LSC · 4219-4933 · 715 bp

Back to Tropaeolum

Pi 0.0751

MarkerSeek score 77.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 53
pi: 0.0751
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAAGGGGTTTGTTGCTGC	55.0	45.0	58.0	58.0	1553-1639 bp	1.0	84.4
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AAAGGGGTTTGTTGCTGCTA	55.0	45.0	58.0	57.9	1551-1637 bp	1.0	84.1
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	AAAAAGGGGTTTGTTGCTGC	50.0	45.0	58.1	58.0	1549-1635 bp	1.0	84.0
trnK-UUU-rps16_p4	TTTGTGCCGATTCGAAAACG	AAAAAGGGGTTTGTTGCTGC	45.0	45.0	58.0	58.0	1640 bp	0.5	59.5
trnK-UUU-rps16_p5	TTTGTGCCGATTCGAAAACG	AAAGGGGTTTGTTGCTGCTA	45.0	45.0	58.0	57.9	1638 bp	0.5	59.2