Hotspot

ycf1

IRb · 113970-115058 · 1089 bp

Back to Trollius

Pi 0.0107

MarkerSeek score 57.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1089
variable_sites: 44
pi: 0.0107
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	GGGGGCGTATCTCCTCTTAT	55.0	55.0	58.0	58.1	1510-1566 bp	1.0	87.7
ycf1_p2	GCTCCGTTCCATGTCTCATT	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.0	58.1	1395-1451 bp	1.0	87.7
ycf1_p3	GTAATGGCTCCGTTCCATGT	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.0	58.1	1401-1457 bp	1.0	87.6
ycf1_p4	ACTCCCGGAACTTCTTCGTA	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.1	58.1	1418-1474 bp	1.0	87.2
ycf1_p5	TCGTAACTCCCGGAACTTCT	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.1	58.1	1423-1479 bp	1.0	87.2