Hotspot

psbT-psbN

LSC · 78484-78565 · 82 bp

Back to Trollius

Pi 0.0405

MarkerSeek score 65.60

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 82
variable_sites: 5
pi: 0.0405
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GCCTCCTAAGGTTCCGACTA	GATCCATTCGAGGAACACGG	55.0	55.0	58.2	58.4	132-140 bp	1.0	80.8
psbT-psbN_p2	TTCGAGAGCCTCCTAAGGTT	GATCCATTCGAGGAACACGG	50.0	55.0	57.8	58.4	139-147 bp	1.0	80.4
psbT-psbN_p3	TTTCGAGAGCCTCCTAAGGT	GATCCATTCGAGGAACACGG	50.0	55.0	57.8	58.4	140-148 bp	1.0	80.4
psbT-psbN_p4	GCCTCCTAAGGTTCCGACTA	GGCAACCCTCTCAACAACTA	55.0	50.0	58.2	57.4	155-163 bp	1.0	78.8
psbT-psbN_p5	TTCGAGAGCCTCCTAAGGTT	GGCAACCCTCTCAACAACTA	50.0	50.0	57.8	57.4	162-170 bp	1.0	78.6