Hotspot

psbE-petL

LSC · 68758-70021 · 1264 bp

Back to Trollius

Pi 0.00796

MarkerSeek score 62.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 35
pi: 0.00796
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAAGCCGCCAGTAGAAAACC	50.0	50.0	57.9	58.8	1371-1412 bp	1.0	78.8
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AAAGCCGCCAGTAGAAAACC	45.0	50.0	58.2	58.8	1380-1421 bp	1.0	77.8
psbE-petL_p3	TGCTGACGAATAACCAACCC	AAAGCCGCCAGTAGAAAACC	50.0	50.0	58.2	58.8	1385-1426 bp	1.0	77.7
psbE-petL_p4	CGAATAACCAACCCGCAATG	AAAGCCGCCAGTAGAAAACC	50.0	50.0	57.8	58.8	1379-1420 bp	1.0	77.7
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AAAGCCGCCAGTAGAAAACC	50.0	50.0	58.2	58.8	1373-1414 bp	1.0	77.3