Hotspot

petA-psbJ

LSC · 67602-67979 · 378 bp

Back to Trollius

Pi 0.0117

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 9
pi: 0.0117
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATCACGTGTCCAAGGTCTT	TGGCTTATAGGCACTGTAGC	50.0	50.0	57.8	57.1	578-595 bp	1.0	74.9
petA-psbJ_p2	CATCACGTGTCCAAGGTCTT	AGGCACTGTAGCTGGTATTC	50.0	50.0	57.8	57.0	570-587 bp	1.0	74.3
petA-psbJ_p3	CATCACGTGTCCAAGGTCTT	GGCACTGTAGCTGGTATTCT	50.0	50.0	57.8	57.0	569-586 bp	1.0	74.3
petA-psbJ_p4	CATCACGTGTCCAAGGTCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	560-577 bp	1.0	73.6
petA-psbJ_p5	CATCACGTGTCCAAGGTCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	561-578 bp	1.0	72.8