Hotspot

ycf4-cemA

LSC · 64905-65737 · 833 bp

Back to Trollius

Pi 0.0186

MarkerSeek score 60.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 71
pi: 0.0186
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	566-991 bp	1.0	89.2
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	AAGGGGTTTTAGCGCTTTCT	45.0	45.0	57.9	57.7	523-948 bp	1.0	88.0
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	AGAAGGGGTTTTAGCGCTTT	45.0	45.0	57.9	57.7	525-950 bp	1.0	88.0
ycf4-cemA_p4	TGCGCGTACCAATTGAAGTA	AGGGGTTTTAGCGCTTTCTT	45.0	45.0	57.9	57.7	522-947 bp	1.0	88.0
ycf4-cemA_p5	TTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.7	58.1	568-993 bp	1.0	87.3