Hotspot

ndhD

SSC · 126996-128498 · 1503 bp

Back to Triumfetta

Pi 0.00266

MarkerSeek score 44.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 12
pi: 0.00266
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACGGGTTTTTCTGGTCCAAG	TCATAGGATTGCTTAGTTTTTGA	50.0	30.4	58.3	54.0	1589 bp	1.0	40.6
ndhD_p2	CGGGTTTTTCTGGTCCAAGT	TCATAGGATTGCTTAGTTTTTGA	50.0	30.4	58.3	54.0	1588 bp	1.0	40.6
ndhD_p3	CACGGGTTTTTCTGGTCCAA	TCATAGGATTGCTTAGTTTTTGA	50.0	30.4	58.6	54.0	1590 bp	1.0	40.6
ndhD_p4	CGGGTTTTTCTGGTCCAAGT	ATCATAGGATTGCTTAGTTTTTGA	50.0	29.2	58.3	54.6	1589 bp	1.0	40.6
ndhD_p5	CGGGTTTTTCTGGTCCAAGT	TCATAGGATTGCTTAGTTTTTGAT	50.0	29.2	58.3	54.6	1588 bp	1.0	40.6