Hotspot

clpP1

LSC · 74394-76562 · 2169 bp

Back to Triumfetta

Pi 0.0026

MarkerSeek score 61.30

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2169
variable_sites: 14
pi: 0.0026
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	GAAGGAAGGGAAAAAGAGGGA	ACGCTTCCACATCAAAGTGA	47.6	45.0	57.2	57.7	2244-2306 bp	1.0	66.6
clpP1_p2	AGAAGGAAGGGAAAAAGAGGG	ACGCTTCCACATCAAAGTGA	47.6	45.0	57.2	57.7	2245-2307 bp	1.0	66.5
clpP1_p3	AAGGAAGGGAAAAAGAGGGA	ACGCTTCCACATCAAAGTGA	45.0	45.0	55.9	57.7	2243-2305 bp	1.0	64.4
clpP1_p4	AAGAAGGAAGGGAAAAAGAGGG	ACGCTTCCACATCAAAGTGA	45.5	45.0	57.9	57.7	2246-2308 bp	1.0	64.1
clpP1_p5	AGTAACCCCTTGCCTTAAGT	ACGCTTCCACATCAAAGTGA	45.0	45.0	56.0	57.7	2267-2329 bp	0.857143	59.5