Hotspot

trnL-UAG-rpl32

SSC · 129963-131179 · 1217 bp

Back to Triumfetta

Pi 0.00646
MarkerSeek score 42.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1217
variable_sites
24
pi
0.00646
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC TTTTGTACAACAAATGAATTTGGAGT 50.0 26.9 58.0 57.0 1271-1281 bp 1.0 41.8
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA TTTTGTACAACAAATGAATTTGGAGT 50.0 26.9 58.0 57.0 1272-1282 bp 1.0 41.8
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA TTTTGTACAACAAATGAATTTGGAGT 50.0 26.9 57.9 57.0 1281-1291 bp 1.0 41.7
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC TTTTGTACAACAAATGAATTTGGAGT 55.0 26.9 57.8 57.0 1280-1290 bp 1.0 41.7
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TTTTGTACAACAAATGAATTTGGAGT 55.0 26.9 57.8 57.0 1279-1289 bp 1.0 41.7