Hotspot

ndhD-ccsA

SSC · 128499-128815 · 317 bp

Back to Triumfetta

Pi 0.0143

MarkerSeek score 58.60

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 317
variable_sites: 9
pi: 0.0143
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	ATCCGGATTTCGTCCTCTCA	GTACATTCCGCAATTGTGGC	50.0	50.0	58.2	58.1	506-526 bp	1.0	79.4
ndhD-ccsA_p2	ATCCGGATTTCGTCCTCTCA	TACATTCCGCAATTGTGGCT	50.0	45.0	58.2	58.2	505-525 bp	1.0	78.8
ndhD-ccsA_p3	ATCCGGATTTCGTCCTCTCA	TTCCGCAATTGTGGCTTCTA	50.0	45.0	58.2	57.8	501-521 bp	1.0	78.6
ndhD-ccsA_p4	ATCCGGATTTCGTCCTCTCA	CATTCCGCAATTGTGGCTTC	50.0	50.0	58.2	58.4	503-523 bp	1.0	77.2
ndhD-ccsA_p5	ATCCGGATTTCGTCCTCTCA	ATTCCGCAATTGTGGCTTCT	50.0	45.0	58.2	58.5	502-522 bp	1.0	76.5